Glycoprotein biosynthesis in developing cerebellar granule cell cultures

Cover of: Glycoprotein biosynthesis in developing cerebellar granule cell cultures | Simone M. Nicholson

Published by National Library of Canada in Ottawa .

Written in English

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SeriesCanadian theses = Thèses canadiennes
The Physical Object
Pagination3 microfiches.
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Open LibraryOL18681837M
ISBN 100315565470

Download Glycoprotein biosynthesis in developing cerebellar granule cell cultures

In the developing cerebellum, there is a burst of glycoprotein expression on the plasma membrane of the parallel fibres associated with the granule cells (Zanetta et al., ).

This phase coincides with an increase in the activity of some glycosidase enzymes (Zanetta et al., ), which may be involved in glycoprotein by: 5.

Cell cultures Cultures enriched in granule cells were prepared from the cerebella of 8-day-old rats according to Thangnipon et Cultures enriched in cerebellar astrocytes were prepared from 8-day-old rats which had been treated 2 days prior to sacrifice with hydroxyurea (2 mg/kg i.p.2"17).Cited by: 3.

MAG, its receptor (NgR1) and co-receptor (p75 NTR) are expressed in the cerebellum. To establish the role of MAG in developing CGNs, we started the investigation by studying the expression pattern of MAG, NgR1 and p75 NTR in CGNs in vivo and in vitro.

MAG was expressed in P2, P4, P7, P10, P14 and P60 cerebellar (Fig. 1a–c).MAG immunoreactivity was detected diffusely along the whole P2 and P4 Cited by: 1. Purkinje Cell Minimum Essential Medium Cerebellar Granule Cell (), Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1.

Microexplant Cultures of the Cerebellum. In: Fedoroff S., Richardson A. (eds) Protocols for Neural Cell Culture. Humana Press, Totowa, NJ Cited by: 4. ABSTRACT The biosynthesis of the neural cell adhesion molecule (N-CAM) was studied in primary cultures of rat cerebral glial cells, cerebellar granule neurons, and skeletal muscle cells.

The three cell types produced different N-CAM polypeptide patterns. The proliferation of cerebellar granule cell precursors (CGCPs) in EGL, their differentiation into cerebellar granule cells (CGCs), and migration of CGCs toward IGL are tempo-spatially modulated [3,10,14].

Cell adhesion proteins, which play an important role in the morphogenesis of cerebellum, include immunoglobulin superfamily (IgSF) proteins. Background: Neurons in the central nervous system (CNS) are generated by symmetric and asymmetric cell division of neural stem cells and their derivative progenitor cells.

Cerebellar granule cells are the most abundant neurons in the CNS, and are generated by intensive cell division of granule cell precursors (GCPs) during postnatal development. Albrechtsen M., Møller-S.P. C.J., Bock E. () Biosynthesis of the Neural Cell Adhesion Molecule (N-CAM) Glial, Muscle and Neuronal Forms of N-CAM Studied in Primary Rat Cell Cultures.

In: Althaus H.H., Seifert W. (eds) Glial-Neuronal Communication in Development and Regeneration. NATO ASI Series (Series H: Cell Biology), vol 2. Several studies reported the biological significance of B3GNT5-mediated synthesis of glycolipids in B-cell activation, 6 pre-implantation development, 7 and development of nerve system, 8, 9 The.

In rodents, cerebellar development continues after birth, characterized by the maturation of granule neurons. Cerebellar granule neurons (CGNs) are the most abundant neuronal type in the central nervous system, and they provide an excellent model for investigating molecular, ­cellular, and physiological mechanisms underlying neuronal.

The biosynthesis of the neural cell adhesion molecule (N-CAM) was studied in primary cultures of rat cerebral glial cells, cerebellar granule neurons, and skeletal muscle cells.

The three cell types produced different N-CAM polypeptide patterns. 1. Introduction. Cerebellar granule cells (CGCs) constitute the vast majority of neurons in the cerebellum.

Therefore, determining the mechanisms underlying the proliferation of cerebellar granule cell precursors (CGCPs) and the differentiation of CGCPs into CGCs is important for understanding cerebellar development.

The biosynthesis of the neural cell adhesion molecule (N-CAM) was studied in primary cultures of rat cerebral glial cells, cerebellar granule neurons, and skeletal muscle cells. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): ABSTRACT The biosynthesis of the neural cell adhesion molecule (N-CAM) was studied in primary cultures of rat cerebral glial cells, cerebellar granule neurons, and skeletal muscle cells.

The three cell types produced different N-CAM polypeptide patterns. Glial cells synthesized aMr polypeptide B. Cerebellar granule cells form the thick granular layer of the cerebellar cortex and are among the smallest neurons in the brain. (The term granule cell is used for several unrelated types of small neurons in various parts of the brain.) Cerebellar granule cells are also the most numerous neurons in the brain: in humans, estimates of their total number average around 50 billion, which means.

Ornithine Decarboxylase Activity During Development of Cerebellar Granule Neurons Article (PDF Available) in Journal of Neurochemistry 71(5) December with 21 Reads. Human Cerebellar Granule Cells (HCGC) Catalog # Cell Specification The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool [1].

Reelin (Reln) is a glycoprotein that in postnatal and adult mammalian brain is believed to be secreted from telencephalic GABAergic interneurons and cerebellar glutamatergic granule neurons into the extracellular matrix.

To address the question of whether Reln neurosecretion occurs via a regulated or a constitutive process, we exposed postnatal rat cerebellar granule neurons (CGNs). Electron microscopic immunocytochemistry revealed that around the peak of cerebellar neuronal migration (7-day-old rat), 9-O-acetyl GD3 was localized at the contact sites between migrating granule cells and radial glia in the external granular layer and prospective molecular layer.

In addition, using microexplant and slice cultures of the. Balázs R., Jørgensen O.S., Hack N., Gallo V., Kingsbury A., Cotman C. () Role of Excitatory Amino Acids in the Development of Cerebellar Granule Neurones.

In: Huether G. (eds) Amino Acid Availability and Brain Function in Health and Disease. NATO ASI Series (Series H: Cell Biology), vol Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1 J.

Kruse 1 R. Mailhammer 1. During CNS development, multipotent neural stem cells give rise first to various kinds of specified precursor cells, which proliferate extensively before terminally differentiating into either neurons or glial cells. It is still not clear, however, whether the specified precursor cells are irreversibly determined to differentiate into their particular cell types.

Effects of a glycoprotein synthesis inhibitor on myelination were investigated in rat cerebellum. The glycoprotein synthesis inhibitor, tunicamycin (TM), was injected intracranially into newborn rats.

The activity of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) in the cerebellum was significantly reduced in 2-week-old animals and was restored to the normal level by age 3 weeks. Kensuke Nakahira's 44 research works with 1, citations and reads, including: Pleiotrophin mRNA is highly expressed in neural stem (progenitor) cells of mouse ventral mesencephalon and the.

Myelin-associated glycoprotein (MAG) binds to the nerve cell surface and inhibits nerve regeneration. The nerve cell surface ligand(s) for MAG are not established, although sialic acid-bearing glycans have been implicated. We identify the nerve cell surface gangliosides GD1a and GT1b as specific functional ligands for MAG-mediated inhibition of neurite outgrowth from primary rat cerebellar.

Cerebellar Granule Neurons (CGNs). Cell isolation and culture were as described (16). Cerebella were removed from postnatal day 4–5 Sprague–Dawley rats and dissociated enzymatically. CGNs were isolated by the two-step purification method of Hatten (17).

Cells at the 35% 60% Percoll interface [. Only when immature (0 div) granule cells were freeze-fixed on the dish before the addition of pontine explants was pontine axon extension inhibited to a similar extent as in sister dishes with live cells (extension on live immature granule cells, ± % of laminin control, n = 35, N = 6, p contr.

As a forward-looking company as well as a leading customer service provider, Creative Biolabs has successfully developed versatile glycoprotein analysis platforms to provide the largest and most diverse portfolio of glycoprotein products or services.

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Cultivation of mouse cerebellar cells in serum free, hormonally defined media: survival of neurons. Neurosci Lett. Mar 5; 28 (3)– Fischer G, Künemund V, Schachner M. Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1.

J Neurosci. MATERIALS AND METHODS. Neuronal Cell Cultures—Cerebellar granule cells were prepared from 7–8-day-old Wistar rats (Iffa Credo, France).Neurons were seeded on poly-l-lysine (50 μg/ml)-coated dishes or coverslips at a density of × 10 6 cells/cm 2 and cultured in Eagle's basal medium (EMEM, Sigma) supplemented with 10% fetal calf serum (Invitrogen), 25 m m KCl, % penicillin-streptomycin.

Many proteins control how cells are organised in your body. For example, a group of proteins called the neurotrophins help to control the life and death of brain cells. After a brain injury, neurotrophins can cause nerve cells to die by working together with another protein called p75NTR.

The p75NTR protein is also found in another type of brain cell called granule progenitor cells. Cerebellar development involves several cell populations, and cellular activities in the developing cerebellum are extremely dynamic.

Cell type specification of precursor cells, rapid cell proliferation, and cell migration/patterning occur during cerebellar development, which are thought to require tremendous energy supply and robust biosynthesis.

Primary cultures of granule neurons from the post-natal rat cerebellum provide an excellent model system for molecular and cell biological studies of neuronal development and function. The cerebellar cortex, with its highly organized structure and few neuronal subtypes, offers.

Analysis of the Nr-CAM/L1 double knockouts suggested defects in granule cells during cerebellar development.

To further test the involvement of Nr-CAM and L1 in granule cell development, we used a dissociated cerebellar culture that mimics the in vivo differentiation of granule and Purkinje cells (Hatten et al., ).

As the double mutant mice. Granule cells were dissociated from early postnatal mouse cerebella and labeled with a fluorescent dye probe PKH Small number of the labeled cells were mixed with cerebellar cortical microexplant cultures or transplanted into cerebellar cortical organotypic explants, and their time‐dependent morphological changes during cultures were examined with fluorescence microscopy.

Importantly, glucosylceramide synthase inhibitors do not directly affect glycoprotein biosynthesis, and P4 does not reduce cell viability or neurite outgrowth (30, 31). Because of the relatively rapid turnover of gangliosides on the surface of CGNs in culture (t 1/2 cell surface gangliosides.

Like immune cells, cultures of cerebellar granule neurons are very homogeneous and provide a unique opportunity for quantifying by flow cytometry one form of programmed cell death in the CNS, the. F ig Monovalent sialoside reverses MAG-mediated inhibition of axon outgrowth in rat cerebellar granule neurons in culture.

Cells were plated on control substrata (A and C) and on the same substrata adsorbed with a mild detergent extract of myelin, containing MAG (B and D).After 48 h in control medium (A and B) or in the same medium containing 10 μ m disialyl T glycoside (C and D), cells.

Neuroserpin is a serine protease inhibitor of the serpin family that has been identified as an axonally secreted glycoprotein in neuronal cultures of chicken dorsal root ganglia. To obtain an indication for possible functions of neuroserpin, we analyzed its expression in the developing and the adult CNS of the mouse.

Purkinje cells, also called Purkinje neurons, are neurons in vertebrate animals located in the cerebellar cortex of the je cell bodies are shaped like a flask and have many threadlike extensions called dendrites, which receive impulses from other neurons called granule cell also has a single projection called an axon, which transmits impulses to the part of the brain.

Purchase Synaptic Constituents in Health and Disease - 1st Edition. Print Book & E-Book. ISBN  To the best of our knowledge, a significant loss of cerebellar granule cells associated with lentiviral infections has only been shown in cell culture [49, 64].

Apart from case reports, Purkinje cell loss in this context has not been systematically investigated yet [65, 66].Briefly, 2 ml of Y cell culture ( 3 cells/ml) in serum-free medium as above plus % ITS were treated with 25 ml of a solution containing PEDF protein in phosphate-buffered saline with 1% BSA.

After 7 days of treatment, the cells were attached to poly-D-lysine-coated plates. The differentiation state of the cells was monitored by.

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